A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS
نویسندگان
چکیده
منابع مشابه
A New Method for Analyzing Electron Microscope Autoradiographs Using Hypothetical Grain Distributions
A new method for the analysis of electron microscope autoradiographs is described which has two advantages over other methods. This method provides estimates for the activity in different regions of the section and takes into account the actual geometrical shape and position of different regions within the section. Use is made of the measured distribution of grains about a thin line source to c...
متن کاملLipid staining for the electron microscope: a new method.
Tissues fixed in osmium tetroxide or in combined osmium and glutaraldehyde (Hinde), embedded in Spurr's medium, cut at 0-5-I mum and mounted in Farrants' gum medium containing ethyl gallate, show good staining of lipid-contaning structures (droplets of triglyceride, membranes, mitochondria, etc.) in the light microscope. Such preparations show moderate contrast in the electron microscope withou...
متن کاملResolution of electron microscope autoradiography. IV. Application to analysis of autoradiographs
The previous publications of this series described the expected grain distributions around model radioactive structures in EM autoradiographs as a function of the specimen resolution. This family of expected distributions was called the "universal curves". In the present study, experiments on 14C-sources were compared, significant differences were found depending on the energy of the isotope. T...
متن کاملElectron microscope study of microtexture and grain surfaces in limestones
Electron micrographs of directcarbon replicas having magnifications of 5400 to 23, 500 times reveal in detail the grain size, shape, and packing of the particles comprising very fine-grained limestones. At these magnifications, however, only portions of a few grains in mediumor coarsegrained limestones can be observed. Grain contacts are mostly curvilinear in very fine-grained limestones but ar...
متن کاملA Method for Intracellular Autoradiography in the Electron Microscope
A technic is described for high resolution intracellular autoradiography in the electron microscope. Cultures of LLC-MK(2) monkey kidney cells were incubated for 72 hours in a medium containing 0.4 microcurie per ml of thymidine-H(3). After labeling, the cells were fixed with osmium tetroxide and embedded in methacrylate. Ultrathin sections of the labeled tissue were taken up on Formvar-coated ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1973
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.57.1.9